The Effect of Oxidation of Filtrates of a Chicken Sarcoma (chicken Tumor I—rous)
نویسنده
چکیده
1. The rapid autoinactivation at 37 degrees of candle filtrates of the Rous Foul Sarcoma I is due in large part to oxidation. 2. Such oxidation may be prevented by a dilution of 1-2000 cysteine hydrochloride brought to pH 7.4 with NaOH. 3. The variability of infectiousness of candle filtrates is due in part at least to oxidative changes which take place during their preparation; and more active extracts may be obtained by preventing these by means of cysteine. 4. For elaborate experiments it is possible to preserve filtrates in the ice box with cysteine and vaseline until test inoculations have established their infectivity.
منابع مشابه
The age of the tumor-bearing hosts as a factor conditioning the transmissibility of the Rous sarcoma by filtrates and cells.
Fundamentally, the present paper is concerned with both the state of the Rous sarcoma virus— masked or free—when the tumor is made to grow either in old or in young hosts, as well as with properties of the tumor itself, associated with the virus in either state. It is well known that the causative viruses of chicken sarcoma can vanish in the most unpre dictable way, so that filtrates from a...
متن کاملA study of three new duck variants of the Rous chicken sarcoma.
Past studies have disclosed the fact that the virus of the Rous sarcoma of chickens can be adapted to alien species of birds provided, first, that young or newborn individuals be injected with the tumor material (2, 3) and second, that this tumor had been grown in chickens of a certain age (4). By fulfilling these requirements, several other duck strains of chicken tumors have been obtained. Th...
متن کاملStudies of the Transmissible Agent of the Rous Chicken Sarcoma I
The viscosity of Rous sarcoma extracts can be reduced with a polysaccharide enzyme isolated from pneumococcus or testis without destruction of the active agent and thus more concentrated active filtrates can be obtained. The active agent can be precipitated with basic proteins. The basic protein can be separated from the components originally coming from the tumor filtrate by electrophoresis.
متن کاملTissue Distribution of the Rous Sarcoma Virus during the Incubation Period
The Rous chicken sarcoma and its causative virus have been intensively studied since Peyton Rous first reported the transmissibility of the tumor by a filtrable agent (10). I t is known that Rous sarcoma virus (RSV) can propagate in tissues other than tumor. Groupe and Rauscher (4) demonstrated progressive propagation in chicken brain after intracerebral inoculation. Groupe et al. (5) found vir...
متن کاملOn the induction of malignant tumors in pigeons by a chicken sarcoma virus after previous adaptation of the virus to ducks.
It was previously reported (5) that cells from duck variants of the Rous sarcoma were effective in inducing malignant growths in adult pigeons inoculated in the breast; this is in contrast to re salts obtained with the original chicken tumor (2). The present .study is concerned with the effect of filtrates front one of the duck variants, and also with characteristic features of the pigeon tumor...
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ورودعنوان ژورنال:
- The Journal of Experimental Medicine
دوره 48 شماره
صفحات -
تاریخ انتشار 2003